ICSI (Intra cytoplasmic sperm Injection) is the best method of fertilization. It is the process of injecting the selected best quality sperm in to the egg ICSI Process involves usage of specialized manipulators and microscope for pickup of selected sperms. The hollow needle is used to inject the selected sperms in to the inner part of the egg (Cytoplasm) Approximately 70 -85% of eggs injected with best quality sperms show evidence of fertilization which is really good.